Biological Science Imaging Resource

FEI Nova 400 Nano SEM

The Nova 400 is a high resolution field emission SEM with through-the-lens differential pumping for low vacuum imaging capabilities.


Resolution @ optimum WD (high vacuum)
1.0 nm @ 15 kV (TLD-SE)
1.8 nm @ 1 kV (TLD-SE)
Resolution @ optimum WD (low vacuum)
1.5 nm @ 10 kV (Helix)
1.8 nm @ 3 kV (Helix)
Accelerating voltage: 200V - 30kV
Probe current: 0.3 pA - 22 nA continuously adjustable
Maximum horizontal field width: 3.9 mm @ 5 mm WD
In-lens SE detector (TLD-SE)
In-lens BSE detector (TLD-BSE)
Everhardt-Thornley SED
Low vacuum SED (LVD)
UHR low vacuum SED (Helix)
TV-rate solid-state BSED
TV-rate low vacuum solid-state BSED (GAD)
STEM detector

The FEI Nova 400 NanoSEM was purchased with funds from NSF grant 0520950