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  • Apoferritin at 1.78 Å resolution.

    Apoferritin

     1.78 Å resolution image of Apoferritin.

  • SEM of shark skin

    SEM of shark skin

    Imaged on FEI Nova 400 NanoSEM

  • TEM of phage virus

    TEM of phage virus

    Imaged on CM120 BT transmission electron microscope

  • Fast frozen, contracting Lethocerus flight muscle

    Fast frozen, contracting Lethocerus flight muscle

    PMID: 20844746 - Imaged on retired FEI CM300-FEG

Biological Science Imaging Resource

We at the Biological Science Imaging Resource (BSIR) are dedicated to making state of the art transmission electron microscopy (TEM) and confocal microscopy available to everyone without multimillion dollar investments in instruments and facilities.  We offer affordable, state of the art, remote robotic TEM imaging using FEI’s Titan Krios™ operated by staff with decades of EM experience. Through the Appion automated data collection system, users can view data collected in real time via a web browser and direct BSIR staff during the data acquisition process. We offer laser scanning dual photon confocal imaging using Zeiss’s LSM 510 confocal microscope which is managed by the physics department.

Many instruments are operated with a user shared collective of knowledge.  Training and technical assistance is provided.  Usage fees help to defray operating expenses incurred by Florida State University. Major equipment purchases are funded through individual and multi-user grants.

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News Feed

CM120BT Status

The CM120BT is operational.  Refresher training is available upon request.   And, cryo sample training is available upon request.   Cryoholder 2 is tested and exhibits a slow pump down time.  Use with caution.  The bake out (heater) controller has a low battery indication that can be bypassed for operation.

 

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Olympus BX61 Status

The BX61 now resides in 336.

Be sure to secure swipe access and a key if you wish to use it after hours.

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HT7800 Status

The Hitachi is OFFLINE.   

The instrument has been repaired and we are currently pumping down the vacuum. 

Regular use may proceed the morning of 11/2.

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Titan status

The titan is offline while we wait for the air handler to be temporarily repaired.  The K3 camera and server are in good shape.  The Apollo camera computer has been returned to the manufacturer for service.

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Nikon CSU-W1 Status

The spinning disc confocal has been moved to 336A and is online.  We are awaiting new operational guidelines.  

When cleaning lenses, stay away from ammonia based cleaners.  BSIR recommends Sparkle.  Look for it near the instrument just for this purpose.  

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