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  • Apoferritin at 1.78 Å resolution.

    Apoferritin

     1.78 Å resolution image of Apoferritin.

  • SEM of shark skin

    SEM of shark skin

    Imaged on FEI Nova 400 NanoSEM

  • TEM of phage virus

    TEM of phage virus

    Imaged on CM120 BT transmission electron microscope

  • Fast frozen, contracting Lethocerus flight muscle

    Fast frozen, contracting Lethocerus flight muscle

    PMID: 20844746 - Imaged on retired FEI CM300-FEG

Biological Science Imaging Resource

We at the Biological Science Imaging Resource (BSIR) are dedicated to making state of the art transmission electron microscopy (TEM) and confocal microscopy available to everyone without multimillion dollar investments in instruments and facilities.  We offer affordable, state of the art, remote robotic TEM imaging using FEI’s Titan Krios™ operated by staff with decades of EM experience. Through the Appion automated data collection system, users can view data collected in real time via a web browser and direct BSIR staff during the data acquisition process. We offer laser scanning dual photon confocal imaging using Zeiss’s LSM 510 confocal microscope which is managed by the physics department.

Many instruments are operated with a user shared collective of knowledge.  Training and technical assistance is provided.  Usage fees help to defray operating expenses incurred by Florida State University. Major equipment purchases are funded through individual and multi-user grants.

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News Feed

CM120BT Status

The CM120BT is operational and the air handler has been repaired.   Cryoholder 2 is tested and exhibits a slow pump down time.  Use with caution.  The bake out (heater) controller has a low battery indication that can be bypassed for operation.

Refresher training is available upon request.       

 

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Olympus BX61 Status

The microscope is now in room 119-I for more space.  The monitor has been upgraded to 1920 x 1200 resolution.

Reservations are accepted and use is logged online now.   Rates remain unchanged.

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HT7800 Status

The instrument is ONLINE.    The camera is operational in 1k x 1k and 1k x 2k format.  New features are now available including quick low resolution images from the Hitachi camera.  We have a Tungsten filament in a the moment while we await  the replacement Lab6.   The replacement 2k x 2k CMOS chip is due to be installed next week.   Remember to turn off the beam and filament at the end of your session.

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Titan status

Titan was down last week due to an autoloader issue which a grid was stuck on the gripper. We have been working with TFS to have engineer to come on-site for service. Meanwhile the Obj. aperture is also malfunctional that stigmatism appears when the aperture is inserted..

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Nikon CSU-W1 Status

The confocal is online.  The software has been updated.  Operational notes updates can be found on the desktop.  Be mindful of the lense you are using and the type of immersion to use with that lense. 

When cleaning lenses, stay away from ammonia based cleaners.  BSIR recommends Sparkle.  Look for it near the instrument just for this purpose. 

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