Bio Unit 1: Room 119P
The FEI CM120 Biotwin is a 120 kV transmission electron microscope primarily used for sample screening. It has a 4k x 4k CCD camera and a cryo stage. It is currently equipped with a tungsten filament and has a resolution of about ~3.4Å.
Specifications:
- 60-120 kV
- TVIPS TemCam- F415MP slow scan bottom-mounted CCD camera with multi-port readout
- 4k x 4k pixels : 15µm pixel size : 61mm x 61mm field of view
- Tietz EM-MENU 3 or EM-MENU 4 software for external PC control of microscope
- Low dose imaging mode
- Cryo stage (liquid nitrogen-cooled cryo blades)
- Compustage (computer controlled goniometer allowing +/- 70 degrees tilt)
- Bright and dark field and diffraction modes
- Tungsten filament e– source
- Magnification 18x – 330,000x
- Resolution ~3.4Å point to point ~2.0 Å lattice image
- Focal length 6.15 mm
Gatan Model 655 Dry Pumping Station
Specimen holders:
- FEI standard high resolution
- FEI double tilt holder
- Gatan Model 670 Ultrahigh tilt analytical holder
- Gatan Model 626 high resolution cryoholder (2)
- Oxford CT 3500 cryoholder
- Gatan CT 3500TR tilt-rotate cryotransfer holder
- Gatan 927 dual orientation tomography holder
The FEI CM-120 was purchased with funds from NSF grant BIR-9512954, NIH grant RR11357 and the Markey Charitable Trust
The Gatan 927 dual orientation tomography holder was purchased with funds from NIH grant S10 RR023564-01.