FEI Titan Krios


Bio Unit 1:  Room 117 

The FEI Titan Krios™ is a state-of-the-art 300 kV transmission electron microscope and imaging robot optimized for high-resolution structural biology.  Through the Appion automated data collection system, users can view data collected in real time via a


  • 300 kV Titan Krios with a third-generation Autoloader
  • Direct Electron Apollo camera : world’s first event-based camera for high-throughput Cryo-EM
  • Gatan BioQuantum K3 camera (5,760 x 4,092) with energy filter
  • FEI Volta Phase Plate : enable imaging at in-focus condition with increased contrast for particles less than 100kD
  • Field compensation system : compensate the small variations of the ambient magnetic fields
  • Leginon and SerialEM: automated data collection
  • Appion: on-the-fly image display and processing
  • Support all types of data collection strategies: Single Particle, Tomography, Tilting data collection, and so on
  • 2.2 Å resolution reconstructed from apoferritin


Submit the following form for Titan reservations:


Leginon Single Particle Protocol:

Images provided by Stagg lab.