LaVision BioTec Ultramicroscope II

King Building: 3034

The LaVision BioTec UltraMicroscope II allows fast 3d imaging of biological systems. It is a flexible and easy solution with clearing protocols, optimized illumination for viewing entire biological systems. It has 6 lightsheets for homogeneous illumination with infinity corrected optics.

Specifications:

Sheet optics
Illumination Uni- and bidirectional
Number of light sheets 1–6
Thickness 4 μm–24 μm
Width 1 mm–20 mm
Numerical aperture 0.0135–0.135
Focus positioning Dynamic
Refractive index matching 1.33–1.56
Zoom microscope Zoom body setup
Zoom Mono zoom
Zoom ratio 0.63×–6.3× (1:10)
Detection optics Zoom body setup Infinity corrected optics setup
Objective lenses 2× 1.1× 4× 12×
Numerical aperture 0.5 0.1 0.3 0.53
FOV diagonal (5.5 Megapixel camera) 1.7 mm–17.6 mm 19.7 mm 5.4 mm 1.8 mm
Total magnification (objective lens + zoom ratio) 1.26×–12.6× (w/o zoom) (w/o zoom) (w/o zoom)
Working distance 5.6 mm (corrected), 10 mm 16 mm, 17 mm 5.6 mm 8.5 mm, 10 mm, 10.9 mm
Refractive index matching 1.33–1.56
Chromatic detection Seven filters
Chromatic correction Dynamic 400 nm–850 nm
Imaging chamber
Imaging solution Aqueous buffers and organic solvents
Sample travel range (X, Y, Z) 1 cm, 1 cm, 1 cm
Sample size μm range to cm range
Chamber size 72 mm × 74 mm × 35 mm
Light source
Laser module Max. 5 laser lines, 50 mW–100 mW per diode
Supercontinuum laser Emission 460 nm–800 nm, 1 mW/nm–3 mW/nm