This training was provided by the manufacture and covers several aspects.  In watching, you will notice several sessions that are a continuation from the previous video.  You will also see different people being trained with different questions and solutions offered.  Speak with other users for questions.  Don't hesitate to contact the company for questions.    Their contact information is posted  on the instrument/monitor/computer.

An alignment guide can be found on the desktop with the pdf linked here.

Ultramicroscope Alignment Protocol