Bio Unit 1:  Room 119T

The BX61 is a research-grade upright microscope, incorporating infinity-corrected optics, motorized Z-axis (focus), and equipped for brightfield, Nomarski DIC, and widefield epi-fluorescence techniques. The Olympus DP71 color digital camera and control so



Specifications:

Olympus UIS2-Series Biological Objectives:



• 1.25x Plan Apo N - 0.04 NA

• 10x U Plan FL N - 0.30 NA

• 20x U Plan FL N - 0.50 NA*

• 40x U Plan S Apo (correction ring) - 0.95 NA*

• 60x Plan Apo N – 1.42 NA oil*

• 100x U Plan S Apo – 1.40 NA oil*



*Nomarski prism for this objective mounted in condenser





Fluorescence Filter Combinations:

  

     Numbers given for excitation (ex) and emission (em) filters are for the center wavelength and width of the bandpass for each, in nanometers



• DAPI  ex: 377/50 em: 447/60

• FITC  ex: 482/35 em: 536/40

• GFP  ex: 473/31 em: 520/35

• Cy3  ex: 531/40 em: 593/40

• Cy5  ex: 628/40 em: 692/40



Olympus DP71 Camera:



• Peltier-cooled 2/3 inch CCD

• 1.45 million pixel full-frame native resolution (1360 x 1024 pixels)

• 12.5 million pixel resolution is achieved by use of piezoelectric pixel shifting technology to produce images having  maximum resolution of 4080 x 3072 pixels