Skip to main content
Florida State University Home Florida State University Home

FSU | Biological Science Imaging Resource

  • Webmail
  • Search FSU
  • Navigation

Main navigation

  • Home
  • Equipment
  • Access
  • Protocols
  • Services
    • Rates
  • Policies
  • About
  • Academics
  • Admissions
  • Research
  • Faculty
  • Students
  • Veterans
  • Support FSU
Biological Science Imaging Resource

Main navigation

  • Home
  • Equipment
  • Access
  • Protocols
  • Services
    • Rates
  • Policies
  • About

Leginon Ptolemy Protocol

General protocols for the instruments within the BSIR

  • Hitachi HT7800 TEM Protocol
  • FEI CM120 BT TEM Protocol
  • Gatan Dry Pumping Station Protocol
  • Titan and Leginon checklist for automated collection
  • Data Transfer / Access Policy
  • Titan Data Collection Policy
  • Cressington HR208 Sputter Coater Protocol
  • Changing the Liquid Nitrogen Tank for the Titan
  • Strategies for Cryo Tilt Series Acquisition
  • Leginon Single Particle Protocol
  • Leginon Tomography Protocol
  • Leginon Tilt Protocol
  • Leginon Ptolemy Protocol
  • Phase plate Protocol
  • Latitude Single Particle Protocol
  • SerialEM Protocol
  • Chameleon document 1
  • Chameleon document 2
  • Chameleon document 3
  • Chameleon document 4
  • Critical point dryer Protocol 

External Links

  • Cryo-EM video course from CalTech

Instrument Status

Chameleon Status

SPTLabtech Chameleon

SPTLabtech Chameleon cryo robot is installed and operational.  Speak to David B. for training or sample prep.

  • Read more about Chameleon Status

GP2 Status

Leica GP2

The Leica GP2 is operational in room 336 of Bio Unit 1.  Speak to David B. for use, scheduling, or training.

  • Read more about GP2 Status

CSU-W1 Status

Full CSU-W1

The Nikon spinning disc confocal in 336A is ONLINE.  Spacial rearrangement allows the door to be fully closed now to limit external light.  The incubator air box has been installed and confirmed operational.

Be sure to use the lenses appropriately (oil vs water immersion).  When cleaning lenses, 'Sparkle' is recommended.  Look for it near the instrument and clean up after yourself.  

  • Read more about CSU-W1 Status

Titan status

Titan

The Titan is ONLINE.  

Our Titan is running today. Users can screen and collect data. For booking, contact Xiaofeng.  Appollo and K3 cameras are good.

  • Read more about Titan status

HT7800 Status

HT7800

The Hitachi is ONLINE and operational.  There is a beam shift when lifting the camera screen above 30K.  The beam shifts back into place within 60-90 seconds.  Service is scheduled this week to correct the issue.

Camera is operational.

During renovations, the TEM is located in Rm 103 of BioUnit 1. This is a shared space.

  • Read more about HT7800 Status

Olympus BX61 Status

BX61

The BX61 is in 336 and is fully operational.

Secure swipe access and a key if you wish to use it after hours.

  • Read more about Olympus BX61 Status
Previous Next

© Biological Science Imaging Resource
Florida State University
89 Chieftan Way, Rm 113A - temporary
Tallahassee, FL 32306

© Florida State University
Tallahassee, FL 32306

FSU Directory Assistance
Questions or Comments

Privacy Policy
Copyright

  • Like Florida State on Facebook
  • Follow Florida State on Instagram
  • Follow Florida State on X
  • Follow Florida State on Youtube
  • Connect with Florida State on LinkedIn
  • More FSU Social Media

Cookie Preferences